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Filaggrin antibody [FLG/1563]

Anti-Filaggrin antibody [FLG/1563] used in Protein Array (Protein Array). GTX17962
Anti-Filaggrin antibody [FLG/1563] used in IHC (Paraffin sections) (IHC-P). GTX17962

Cat. No. GTX17962

Reference 1

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 FLG/1563

Isotype

 IgG2b

Application

 IHC-P, Protein Array

Reactivity

 Human
Package
100 μg ($529)
See all Filaggrin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
IHC-P 1-2μg/ml for 30 minutes at RT
Protein Array Assay dependent

Note :

IHC-P
Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95ºC followed by cooling at RT for 20 minutes.
Not tested in other applications.

Calculated MW

435 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

10mM PBS, 0.05% BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant human Filaggrin protein fragment (around aa 998-1104) (exact sequence is proprietary)

Purification

Protein A/G purified

Conjugation

Unconjugated

RRID

AB_2887570

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ATOD2 , FLG , filaggrin , Filaggrin

Cellular Localization

Cytoplasmic granule

Background

The protein encoded by this gene is an intermediate filament-associated protein that aggregates keratin intermediate filaments in mammalian epidermis. It is initially synthesized as a polyprotein precursor, profilaggrin (consisting of multiple filaggrin units of 324 aa each), which is localized in keratohyalin granules, and is subsequently proteolytically processed into individual functional filaggrin molecules. Mutations in this gene are associated with ichthyosis vulgaris.[provided by RefSeq, Dec 2009]

Database

Research Area

DATA IMAGES

Anti-Filaggrin antibody [FLG/1563] used in Protein Array (Protein Array). GTX17962

GTX17962 Protein Array Image

Analysis of Protein Array containing more than 19,000 full-length human proteins using Filaggrin Mouse Monoclonal Antibody (FLG/1563). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD’s) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD’s) between the Z-score. S-score therefore represents the relative target specificity of a Monoclonal Antibody to its intended target. A Monoclonal Antibody is considered to specific to its intended target if the Monoclonal Antibody has an S-score of at least 2.5. For example, if a Monoclonal Antibody binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that Monoclonal Antibody to protein X is equal to 29.

Anti-Filaggrin antibody [FLG/1563] used in IHC (Paraffin sections) (IHC-P). GTX17962

GTX17962 IHC-P Image

IHC-P analysis of human skin tissue using GTX17962 Filaggrin antibody [FLG/1563].

REFERENCE

Application Reference

Kim BE et al. J Invest Dermatol 2019; (Epub) Side-by-side Comparison of Skin Biopsies and Skin Tape Stripping Highlights Abnormal Stratum Corneum in Atopic Dermatitis.
Application : IHC-P
Reactivity : Human
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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 529